Figure 7: The AUX1 gene has enhanced H3K9/K18 acetylation levels in snl1snl2 seeds.

(a) A schematic diagram of the AUX1 gene structure. The dashed line indicates the ∼500 bp promoter sequence and the black line between the vertical dashes represents the open reading frame of the gene from start codon (ATG) to stop codon (TGA). The relative positions of the PCR-amplified fragments (1–7) for each tested region are depicted below the gene structure. Scale bar represents 500 bp. (b) ChIP analysis of H3K9/K18 acetylation levels on AUX1. Immunoprecipitates were obtained from 12 h imbibed seeds of Col and snl1snl2 using H3K9/K18ac antibody. The accumulation of PCR product after immunoprecipitation has been normalized to ACTIN8. The bars indicate s.e.’s of three biological replicates. The numbers on the x axis represent the PCR-amplified sites described in a. (c) ChIP analysis of 3 × FLAG-SNL1 levels on AUX1. Immunoprecipitates were obtained from 7-day-old seedlings of Col and 35Spro::FLAG-SNL1/snl1snl2 lines (1-6 and 2-6) using a FLAG antibody. Relative amounts of the PCR products were calculated and normalized to ACTIN8, and the value in Col was set as one. The bars indicate s.e.’s of three biological replicates.