Figure 3: Plasmids do not undergo DNA rearrangment during transfection.
From: A segmental genomic duplication generates a functional intron
(a) Schematic of DNA mini-gene constructs used in transfections as described in Figure 2a. Restriction sites are shown along with sizes of DNA digestion fragments. (b) Agarose gels showing diagnostic digests of DNA plasmids recovered from HEK 293 cells transfected with ATP2A2 duplicated 8/9 with insert (D). Control digests from untransfected plasmids are shown; ATP2A2 duplicated 8/9 with insert (D), ATP2A2 single (C1) and ATP2A2 single with 6 bp insert (C2), along with DNA size markers (m). All the recovered DNA plasmids were the same size as the transfected DNA plasmid ATP2A2 duplicated 8/9 with insert (D).
