Figure 8: Myogenic potential of isolated AP-derived YFP+ cells.

(a) FACS analysis with a gate selected to sort YFP+ cells. (b) Relative frequency of double-positive YFP+/Pax7+ cells as % of the total YFP+ cells, obtained immediately after sorting, at different time points after tamoxifen induction and after cardiotoxin injection (CTX). Error bars indicate standard deviation. Results were assessed for statistical significance by Student's t-test (unpaired, two-tailed), and differences were considered statistically significant at P<0.05. Asterisks in the histogram indicate the different P values: ***P<0.001. (c–e) Freshly isolated YFP+ cells were stained with anti-GFP antibody (green) and anti-Pax7 (red). Scale bar, 20 μm (f) Skeletal myogenic YFP+ clone, stained with anti-MyHC (red), anti-GFP (green) and DAPI (blue). (g) Smooth myogenic YFP+ clone, stained with anti-SMA (red), anti-GFP (green) and DAPI (blue). (h,i) Smooth myogenic (h) and mixed (i) YFP+ clones stained for anti-MyHC (green), anti-SM22α (magenta) and DAPI (blue). We counted 2 double-positive clones on a total number of 114 clones scored. Scale bars, 40 μm.