Figure 4: Assembly of FtsZ on monolayers seeded with FtsA. | Nature Communications

Figure 4: Assembly of FtsZ on monolayers seeded with FtsA.

From: Escherichia coli FtsA forms lipid-bound minirings that antagonize lateral interactions between FtsZ protofilaments

Figure 4

(a) Mock experiment in which 5 μM FtsZ is added to monolayers, but no FtsA was present; (b) 0.1 μM FtsA+5 μM FtsZ; (c) 0.5 μM FtsA+5 μM FtsZ; (d) 0.5 μM FtsA+5 μM FtsZ on a grid without a monolayer. (e–g) Same conditions as (c), showing examples of aligned unbundled FtsZ filaments (long arrows) on FtsA minirings merging with bundled FtsZ filaments not associated with visible FtsA minirings (short arrows). (h) A large swirl of aligned FtsZ filaments on FtsA minirings. ATP and GTP were added to all experiments. Scale bar, 100 nm.

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