Table 1 Summary of gene-targeting assays in LIG4−/−POLQ−/− cells.
From: Dual loss of human POLQ and LIG4 abolishes random integration
Target locus | Targeting vector | No. of cells treated ( × 106) | Gene-targeting efficiency | ||
|---|---|---|---|---|---|
5′-arm | 3′-arm | Selection marker* | |||
HPRT | 3.8 kb | 5.1 kb | PPGK -Neo | 40.0 | 100% (138/138) |
HPRT | 1.7 kb | 2.8 kb | 2A-Puro | 24.0 | 100% (59/59) |
AAVS1 | 4.4 kb | 3.1 kb | PPGK -Neo | 4.0 | 100% (8/8) |
PRKDC | 2.1 kb | 5.1 kb | PPGK -Hyg | 8.0 | 100% (3/3) |
RAD54B | 3.3 kb | 4.5 kb | PPGK -Hyg | 8.0 | 100% (5/5) |
RAD54L | 2.9 kb | 3.6 kb | 2A-Puro | 8.0 | 100% (1/1) |
TP53BP1 | 0.9 kb | 1.5 kb | IRES-Hyg | 8.0 | 100% (2/2) |
