Figure 4: Measurements on multiple length scales by msFCCS.
From: Retrieving the intracellular topology from multi-scale protein mobility mapping in living cells
(a) Line-confocal fluorescence images of representative U2OS cells expressing GFP1, GFP3 and GFP5. The line positions for the measurement of AC and spatial XC functions in homogeneous regions of the nucleus and cytoplasm are indicated. (b) Representative average AC (0 μm) and XC (1.6 and 3 μm) curves obtained in cytoplasm (red) and nucleus (blue). For large diffusion distances, distinct peaks were visible in the diffusion time distributions that shift to larger translocation times for increasing diffusion distance. Solid lines represent the fitted model functions given by Supplementary Equation (22). Scale bar, 5 μm. Cyt, cytoplasm; Nuc, nucleus; Norm, normalized.
