Table 2 Potential off-target sites with one to three mismatches to gRNA and their observed-targeting efficiencies.

From: Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells

Target and off-target indel frequencies

Number of mismatches

Number of genomic sites

Cas9 targeting efficiency

0

1

53.9%

1

0

—

2

0→1

36.7%

3

32

~0.15% per site

  1. The variant allele of Chr5_OT represents the only site with only two mismatches to the gRNA and Cas9 target efficiency at this site was observed to be 36.7%, comparable with that of the target site (53.9%); the remaining sites with three mismatches were targeted by Cas9 with an average efficiency of ~0.15% (Supplementary Table 2) as measured by deep sequencing.
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