Figure 5: Tissue-specific Glucome RNAi screening of 213 non-NHR class transcription factors.

(a,b) Transcription factors (213) tested in the primary screen, loss of 26 genes in the fat body (a) and 32 genes in the muscle (b) significantly increased haemolymph glucose; n=4 each (⩾10 larvae per replicate), statistical significance was evaluated as outlined in Fig. 3. Initially assessed by two-tailed Student’s t-test (P<0.05, error bars indicate s.e.m.). # indicates a common hit in fat body and muscle screens. Hits were reclassified based on P values from the Wilcoxon rank-sum test, as in Fig. 3. High confidence (in magenta): P<0.05, medium confidence (in blue): P=0.057, and low confidence (in grey): P>0.057. Genes are alphabetically listed in each confidence level. After the primary screen, three repeat glucose fold changes for hyperglycaemia candidates were assessed by a 95% confidence interval; Ф symbol above bar indicates the genes not validated with 95% confidence. (c) The proportion of hyperglycaemia transcription factor hits in either tissue, overlapping hits in both tissues and genes previously associated with glucose metabolism or diabetes, and novel genes are indicated.