Abstract
STUDIES1–6 of the molecular nature of antibiotic resistance (R) factors in Escherichia coli have shown that several of them consist of covalently closed molecules of circular DNA. Use was made of this property in the separation of the R factor from chromosomal DNA of E. coli, which has a similar nucleotide-base composition. In selecting for covalently circular R factor DNA molecules, however, the procedures used in these earlier experiments-caesium chloride-ethidium bromide centrifuga-tion7 and bulk nitrocellulose adsorption2—necessarily selected against isolation of other (non-circular) forms of R factor DNA that might have been present.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Nisioka, T., Mitani, M., and Clowes, R., J. Bact., 97, 376 (1969).
Cohen, S. N., Bact. Proc., 69, 49 (1969).
Cohen, S. N., and Miller, C. A., Nature, 224, 1273 (1969).
Cohen, S. N., and Miller, C. A., J. Mol. Biol., 50, 671 (1970).
Falkow, S., Haapala, D. K., and Silver, R. P., in Bacterial Episomes and Plasmids, 136 (Churchill, London, 1969).
Silver, R. P., and Falkow, S., J. Bact., 104, 331 (1970).
Radloff, R., Bauer, W., and Vinograd, J., Proc. US Nat. Acad. Sci., 57, 1524 (1967).
Adler, H. I., Fisher, W. D., Cohen, A., and Hardigree, A. A., Proc. US Nat. Acad. Sci., 57, 321 (1967).
Roozen, K. J., Fenwick, R. J., jun., Levy, S., and Curtiss, R., III, Genetics, 64, S54 (1970).
Inselburg, J., J. Bact., 102, 642 (1970).
Levy, S. B., Nature, 227, 606 (1970).
Kass, L. R., and Yarmolinsky, M. B., Proc. US Nat. Acad. Sci., 66, 815 (1970).
Cohen, S. N., Silver, R. P., Sharp, P. A., and McCoubrey, A. E., Ann. NY Acad. Sci., 182, 172 (1971).
Watanabe, T., and Fukasawa, T., J. Bact., 88, 922 (1964).
Meynell, E., and Datta, N., Genet. Res., 7, 134 (1966).
Cohen, A., Fisher, W. D., Curtiss, R., III, and Adler, H. I., Proc. US Nat. Acad. Sci., 61, 61 (1968).
Hudson, B., and Vinograd, J., Nature, 216, 647 (1967); Clayton, D. A., and Vinograd, J., ibid., 216, 652 (1967).
Piko, L., Blair, D., Tyler, A., and Vinograd, J., Proc. US Nat. Acad. Sci., 57, 838 (1968).
Kontomichalou, P., Mitani, M., and Clowes, R. C., J. Bact., 104, 34 (1970).
Davis, R. W., Simon, M., and Davidson, N., in Methods in Enzymology (Academic Press, New York, in the press).
Cohen, S. N., and Miller, C. A., Proc. US Nat. Acad. Sci., 67, 510 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
COHEN, S., SILVER, R., MCCOUBREY, A. et al. Isolation of Catenated Forms of R Factor DNA from Minicells. Nature New Biology 231, 249–252 (1971). https://doi.org/10.1038/newbio231249a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/newbio231249a0
This article is cited by
-
Visualization of membrane-associated R-plasmid DNA in fraction ofEscherichia coli minicell lyzate
Folia Microbiologica (1980)
-
Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli
Nature (1975)
-
Proteins synthesized by a non-induced bacteriocinogenic factor in minicells of Escherichia coli
Molecular and General Genetics MGG (1972)