Abstract
The development and maturation of semi-invariant natural killer T cells (iNKT cells) rely on the recognition of self antigens presented by CD1d restriction molecules in thymus. The nature of the stimulatory thymic self lipids remains elusive. We isolated lipids from thymocytes and found that ether-bonded mono-alkyl glycerophosphates and the precursors and degradation products of plasmalogens stimulated iNKT cells. Synthetic analogs showed high potency in activating thymic and peripheral iNKT cells. Mice deficient in the peroxisomal enzyme glyceronephosphate O-acyltransferase (GNPAT), essential for the synthesis of ether lipids, had significant alteration of the thymic maturation of iNKT cells and fewer iNKT cells in both thymus and peripheral organs, which confirmed the role of ether-bonded lipids as iNKT cell antigens. Thus, peroxisome-derived lipids are nonredundant self antigens required for the generation of a full iNKT cell repertoire.
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Acknowledgements
We thank W. Just (University of Heidelberg) for Gnpat−/− mice; J. Mattner (Cincinnati Children's Hospital) for S. paucimobilis; P. Seeberger (Max Plank Institute of Colloids and Interfaces, Potsdam) for GSL-1; E. Palmer, K. Karjalainen and P. Dellabona for discussions; P. Cullen for proofreading the manuscript; and L. Angman for technical assistance. Supported by the Swiss National Foundation (3100AO-122464/1 and Sinergia CRS133-124819 to G.D.L., and Oncosuisse to L.M. and G.D.L.) and the Yunnan High-End Technology Professionals Introduction Program (2010CI117 to C.X.).
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F.F. designed and did most of the experiments and participated in manuscript preparation; G.S.R. obtained and interpreted MS data; M.L. did experiments and participated in discussions; S.S. prepared and purified lipid extracts; M.C. and M.K. did experiments; S.F.-P. and J.B. provided genotyped Gnpat−/− mice; G.N. and C.X. provided synthetic compounds; A.C. and A.S. did experiments; L.M. supervised the work, contributed to discussions and prepared the manuscript; and G.D.L. conceived of the experiments, supervised the work and prepared the manuscript.
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Facciotti, F., Ramanjaneyulu, G., Lepore, M. et al. Peroxisome-derived lipids are self antigens that stimulate invariant natural killer T cells in the thymus. Nat Immunol 13, 474–480 (2012). https://doi.org/10.1038/ni.2245
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DOI: https://doi.org/10.1038/ni.2245
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