Abstract
A key step in gene therapy is the efficient transfer of genes in a cell type– and tissue-specific manner. To better understand the mechanism of adeno-associated virus serotype 6 (AAV6) transduction, we used comparative gene analysis (CGA) combined with pathway visualization software to identify a positive correlation between AAV6 transduction and epidermal growth factor receptor (EGFR) expression. Subsequent experiments suggested that EGFR is necessary for vector internalization and probably functions as a co-receptor for AAV6.
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Acknowledgements
We would like to thank B. Handelman for expertise in AAV production, M. Kunkel and S. Holbeck for assistance with the National Cancer Institute Developmental Therapeutics Program and the COMPARE program and T. Jovanka for philosophical guidance.
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M.L.W. performed experiments, analyzed data and wrote the paper; P.A. performed experiments, analyzed data and wrote the paper; M.S. performed experiments and analyzed data; P.A.W. developed software used in CGA; J.S.G. contributed tumor model and head-and-neck tumor cell lines and analyzed data; J.A.C. analyzed data and wrote the paper. All authors were involved in the review and editing of the paper.
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Weller, M., Amornphimoltham, P., Schmidt, M. et al. Epidermal growth factor receptor is a co-receptor for adeno-associated virus serotype 6. Nat Med 16, 662–664 (2010). https://doi.org/10.1038/nm.2145
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DOI: https://doi.org/10.1038/nm.2145
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