Abstract
Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.
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Acknowledgements
We thank T. Wandless (Stanford University) for providing the shield-1 ligand and plasmids encoding ddFKBP and S. Jonjic (University of Rijeka) for monoclonal antibody Croma 101. We thank P. Kay-Jackson for critical reading of the manuscript. This work was in part supported by a grant of the Deutsche Forschungsgemeinschaft (collaborative research grant 587, individual project A13).
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E.M.B. conceived the study and together with M.M. designed experiments and wrote the manuscript. E.M.B., K.W., M.G. and A.B. performed experiments and analyzed data. M.G. assisted in writing the manuscript.
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Glaß, M., Busche, A., Wagner, K. et al. Conditional and reversible disruption of essential herpesvirus proteins. Nat Methods 6, 577–579 (2009). https://doi.org/10.1038/nmeth.1346
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DOI: https://doi.org/10.1038/nmeth.1346
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