Supplementary Figure 14: Illustrative example of the mCitrine intensity plotted versus the mCerulean intensity in HEK293 cells expressing the sensor.
From: A sensor for quantification of macromolecular crowding in living cells
Determination of the slope yields the mCitrine/mCerulean ratio. Crucially, the linearity shows that the ratio is independent of sensor concentration. The R2 values are generally higher than 0.99.
