Supplementary Figure 15: Comparison of the increase in the percent Ficoll equivalent in E. coli calculated from the volume decrease with the value determined using the crowding sensor.
From: A sensor for quantification of macromolecular crowding in living cells
The volume decrease was estimated from the length and width of the bacteria before and after the osmotic upshift and assuming a cylindrical shape of the cells. The % Ficoll equivalents after addition of 125 mM NaCl was subsequently calculated by multiplying the relative volume change with the % Ficoll equivalents before the addition of salt. The % Ficoll equivalents before addition of salt was determined using the crowding sensor and the calibration in Supplementary Figure 12. Median values of 5 independent experiments ± standard deviations are depicted.
