Supplementary Figure 10: User guide for aTF redesign.
From: Engineering an allosteric transcription factor to respond to new ligands
A detailed flowchart that guides the user through the choice of mutagenesis methods based on the choice of the target ligand for aTF redesign. We offer general guidelines on what we consider acceptable fold induction and specificity values by target and native ligands, presented as proportion of WT aTF induction, after the two-stage enrichment screen and following activity maturation. These guidelines could be adjusted on a case-by-case basis depending on the number and quality of ligand-responsive variants after the two-stage enrichment screen, and the nature of downstream application.
