Abstract
Retinal bipolar cells have been assumed to generate purely graded responses to light. To test this idea we imaged the presynaptic calcium transient in live zebrafish. We found that ON, OFF, transient and sustained bipolar cells are all capable of generating fast 'all-or-none' calcium transients modulated by visual stimulation.
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References
Masland, R.H. Nat. Neurosci. 4, 877–886 (2001).
Protti, D.A., Flores-Herr, N. & von Gersdorff, H. Neuron 25, 215–227 (2000).
Palmer, M.J. J. Physiol. (Lond.) 572, 747–762 (2006).
Dreosti, E., Odermatt, B., Dorostkar, M. & Lagnado, L. Nat. Methods 6, 883–889 (2009).
Dorostkar, M.M., Dreosti, E., Odermatt, B. & Lagnado, L. J. Neurosci. Methods 188, 141–150 (2010).
Neves, G., Gomis, A. & Lagnado, L. Proc. Natl. Acad. Sci. USA 98, 15282–15287 (2001).
Burrone, J., Neves, G., Gomis, A., Cooke, A. & Lagnado, L. Neuron 33, 101–112 (2002).
Burrone, J. & Lagnado, L. J. Physiol. (Lond.) 505, 571–584 (1997).
Pan, Z.H. & Hu, H.J. J. Neurophysiol. 84, 2564–2571 (2000).
Zenisek, D., Henry, D., Studholme, K., Yazulla, S. & Matthews, G. J. Neurosci. 21, 4543–4550 (2001).
Cui, J. & Pan, Z.H. Vis. Neurosci. 25, 635–645 (2008).
Acknowledgements
We would like to thank all of the members of the Lagnado laboratory for discussions that contributed to this work. We also thank the Wellcome Trust for funding (grant 083220).
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Experiments were designed by E.D., F.E. and L.L. and performed by E.D., F.E. and L.L. Analysis was carried out by E.D., F.E. and L.L. The manuscript was written by F.E., T.B. and L.L.
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Dreosti, E., Esposti, F., Baden, T. et al. In vivo evidence that retinal bipolar cells generate spikes modulated by light. Nat Neurosci 14, 951–952 (2011). https://doi.org/10.1038/nn.2841
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DOI: https://doi.org/10.1038/nn.2841
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