Abstract
The complexity of the central nervous system calls for immunocytochemical procedures that allow target proteins to be localized with high precision and with opportunities for quantitation. Immunogold procedures stand out as particularly powerful in this regard. Although these procedures have found wide application in the neuroscience community, they present limitations and pitfalls that must be taken into account. At the same time, these procedures offer potentials that remain to be fully realized.
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Acknowledgements
We thank G. Lothe and C. Knudsen for technical assistance, P. Laake for comments, and D. Frydenlund for providing Figure 5. We gratefully acknowledge support from the Norwegian Research Council, the European Union framework programmes and the Letten Fund.
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Amiry-Moghaddam, M., Ottersen, O. Immunogold cytochemistry in neuroscience. Nat Neurosci 16, 798–804 (2013). https://doi.org/10.1038/nn.3418
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DOI: https://doi.org/10.1038/nn.3418
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