Supplementary Figure 6: Thigmotaxis cannot account for improved pattern matching over time.
From: Patterns across multiple memories are identified over time
(a) In the first set of experiments (Fig. 1), mice spent more time in the 10 cm outer ring of the pool during the probe test following a 30 d delay (Mann-Whitney U-test: ranksum = 2345, P = 9.7 x 10–5). (b) This did not account for the reduction in DKL observed in these groups, though, because there was no correlation between time spent in the outer 10 cm zone and DKL for either the 1 d (Pearson's: r = –0.16, P = 0.26) or 30 d (Pearson's: r = 0.15, P = 0.37) groups and there was no difference between the groups in this level of correlation (z-test: z = 0.31, P = 0.32). (c) Thigmotaxis was further dissociated from DKL by the fact that the mice trained on a single platform also showed increased time spent in the outer 10 cm ring of the pool (t-test: t21 = –3.67, P = 0.001), yet they did not show any reduction in DKL (Supplementary Fig. 5b). (d) Thigmotaxis could also not account for the results in the weighted bimodal distribution experiments (Fig. 2). While mice showed improved pattern matching at the 30 d delay, there was no significant difference in thigmotaxis in the probe tests 1 d vs. 30 d after training (Mann-Whitney U-test: ranksum = 125, P = 0.16). Data shown is individual mice or mean ± s.e.m. (**: P < 0.005)
