Supplementary Figure 3: Correlated localization of MADM-labeled RGPs at the embryonic stage and clones at the more mature stage.
From: Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus
(a) Quantification of the average number of neurons in clones labeled at E10 and examined at E15 or P21 (E10-15, n=23; E10-P21, n=23). Data are presented as median with interquartile range and whiskers are the minimum and maximum. *, p=0.03 (Mann- Whitney test). (b) Confocal images of an E14 MADM-labeled thalamus treated with TM at E12 and stained for EGFP (green) and tdTomato (red), and with DAPI (blue). A two-cell local cluster without any radial glial progenitors (boxed area) is labeled in the developing thalamus. High magnification image is shown to the right. M, medial; L, lateral. Scale bars: 100 μm and 10 μm. (c) Quantification of the localizations of MADM-labeled RGPs at the embryonic stage and clones at the more mature stage. Note a clear correlation between the localizations of labeled progenitor cells and labeled clones at different embryonic stages. pTH-R: rostral progenitor domain of the thalamus; PTh: prethalamus. (d) NND analysis of clones labeled at different embryonic stages. Note that the cumulative frequency of NND of clones labeled at E9 (red, n=18), E10 (green, n=28), E11 (orange, n=19), and E12 (blue, n=11) is similar and significantly left-shifted than that of random simulated dataset (gray). Data are presented as mean±s.e.m. n.s., not significant; ****, p=1x10–15 (unpaired t test with Welch's correction). (e) Quantification of the fraction of clones containing neurons only (N) or neurons and glia (N+G) at different embryonic stages.
