Supplementary Figure 11: Shh signaling does not affect the spatial distribution of thalamic clones in the medial ventral posterior region.
From: Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus
(a) Representative 3D reconstructed images of the thalamic hemispheres containing a MADM-labeled clone in the medial ventral/posterior region of the wild type control (top) and SmoM2 (bottom) mice. Note that the clone in the SmoM2 mouse is similarly dispersed radially to that in the control mouse. Scale bar: 500 μm. (b) Quantification of the average number of neurons in green/red G2-X clones labeled at E10 (control, n=23; SmoM2, n=11), E11 (control, n=13; SmoM2, n=10), and E12 (control, n=9; SmoM2, n=3) between the control and SmoM2 mice. Data are presented as median with interquartile range, and whiskers are the minimum and maximum. n.s., not significant. (c) Quantification of the average number of neurons in clones located in the anterior (control, n=9; SmoM2, n=5), medial dorsal (control, n=18; SmoM2, n=6), and medial ventral/posterior (control, n=33; SmoM2, n=21) regions of the P4 control and SmoM2 mice. Data are presented as median with interquartile range, and whiskers are the minimum and maximum. n.s., not significant. (d) Representative in situ hybridization images of the anterior (top) and medial (bottom) section of the control (left) and SmoM2 (right) thalami for RORα. Note the expansion of RORα expression to the anterior region, as well as dorsally, in the SmoM2 thalamus compared to the control. Scale bar: 200 μm. (e) NND analysis of MADM-labeled neuronal clones in the SmoM2 thalamus. Note that compared to random datasets simulated 100 times (gray), the cumulative frequency of NND of thalamic neuronal clones (red, n=31) is significantly left-shifted towards shorter distances, indicating a spatial clustering. Data are presented as mean±s.e.m. ****, p p=10–15 (unpaired t test with Welch’s correction).
