Abstract
The spatial organization of genomes is studied using microscopy- and chromosome conformation capture (3C)-based methods. The two types of methods produce data that are often consistent, but there are cases where they appear discordant. These cases provide opportunities to derive better models of chromatin folding, which can reconcile the datasets.
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References
Wang, S. et al. Spatial organization of chromatin domains and compartments in single chromosomes. Science 353, 598–602 (2016).
Williamson, I. et al. Spatial genome organization: contrasting views from chromosome conformation capture and fluorescence in situ hybridization. Genes Dev. 28, 2778–2791 (2014).
Giorgetti, L. & Heard, E. Closing the loops: 3C versus DNA FISH. Genome Biol., 17 215 (2016).
Fudenberg, G. & Imakaev, M. FISH-ing for captured contacts: towards reconciling FISH and 3C. bioRxiv http://dx.doi.org/10.1101/081448 (2016).
Acknowledgements
The author thanks G. Fudenberg, M. Imakaev, L. Mirny and members of the Dekker laboratory for discussions and sharing insights.
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Dekker, J. Mapping the 3D genome: Aiming for consilience. Nat Rev Mol Cell Biol 17, 741–742 (2016). https://doi.org/10.1038/nrm.2016.151
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DOI: https://doi.org/10.1038/nrm.2016.151
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