Phosphoribosylanthranilate isomerase (PRAI) and indole-3-glycerol phosphate synthase (IGPS) are distantly related α/β-barrel enzymes that catalyze successive steps in tryptophan biosynthesis. A ‘new’ PRAI was evolved from an ‘old’ IGPS by random mutagenesis followed by DNA shuffling and in vivo selection.
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Gerlt, J. New wine from old barrels. Nat Struct Mol Biol 7, 171–173 (2000). https://doi.org/10.1038/73249
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DOI: https://doi.org/10.1038/73249
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