Using a previously developed method, Singer and co-workers have now labeled RNA transcripts and quantitatively measured the synthesis of RNA in living cells. They find that only a small fraction of the RNA polymerases that bind the promoter actually produce messenger RNA. They also observed much faster elongation than previously reported, along with frequent long pauses.
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Lamond, A., Swedlow, J. RNA polymerase II transcription in living color. Nat Struct Mol Biol 14, 788–790 (2007). https://doi.org/10.1038/nsmb0907-788
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DOI: https://doi.org/10.1038/nsmb0907-788
