Fig. 5: Applications of scRNA-seq computational approaches.
From: Single-cell RNA sequencing technologies and bioinformatics pipelines
Cells are living in a dynamic context interacting with their surrounding environment. PCA can be used to identify known and unknown cell clusters. Cell hierarchy reconstruction can be performed after 2D projection of normalized gene expression profiles. Decoding the regulatory network integrates pseudotime-inferred trajectories and clustering gene expression information in 2D space
