Fig. 4

Subclonal mutations: a Stacked bar chart of the clonality status of the top 11 mutated genes in our cohort: bars refer to the left Y-axis and plotted is the proportion of variants that are subclonal (orange), clonal (dark blue) and presumed clonal (light blue)—i.e., cases where confidence intervals of aVAF are all overlapping and low tumor purity could lead to over-estimation of clonality. The yellow line refers to the right Y-axis and represents, for each gene, the ratio between subclonal and clonal variants, i.e., higher values correspond to genes with more subclonal variants. (b)Kaplan–Meier plot of progression-free survival in patients with subclonal (red) or clonal (blue) TP53 mutations with p-values from univariate analysis (log-rank test) adjusted for multiple hypotheses testing. c Kaplan–Meier plot of overall survival in patients with subclonal (red) or clonal (blue) TP53 mutations with p-values from univariate analysis (log-rank test) adjusted for multiple hypotheses testing