Fig. 5: Performance of HiFi-SIM on reconstructing raw data with low modulation depth and strong background.
From: High-fidelity structured illumination microscopy by point-spread-function engineering
a Equivalent wide-field image of microtubules in live U2OS cells with strong background fluorescence is shown in the left, and the SR images reconstructed using fairSIM and HiFi-SIM. Lower-right corner shows corresponding reconstructed spectrum. b–d Magnified images of the red-box, cyan-box, and magenta-box regions in a. e–g Intensity profiles along the red, cyan, and magenta lines in b–d, respectively
