Fig. 6: Imaging of vesicle in live U2OS cells. | Light: Science & Applications

Fig. 6: Imaging of vesicle in live U2OS cells.

From: High-fidelity structured illumination microscopy by point-spread-function engineering

Fig. 6

a Comparison of SR images reconstructed by fairSIM (left), Hessian-SIM (middle), and HiFi-SIM (right). b Magnified SR images of vesicles with different sizes using fairSIM (top), Hessian-SIM (middle), and HiFi-SIM (bottom). Note that vesicles with larger sizes reconstructed by fairSIM have obvious defocused background artifacts in the center and outside. Further performing the Hessian denoising procedure on the results of fairSIM can suppress the artifacts to a certain extent, but cause the center of the vesicles to be blurred. c Intensity profiles along the red line in b

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