Fig. 7 | Mucosal Immunology

Fig. 7

From: Bone morphogenetic protein 6 (BMP-6) modulates lung function, pulmonary iron levels and cigarette smoke-induced inflammation

Fig. 7

Effect of CS exposure and BMP-6 deficiency on chemokines/cytokines in lung tissue and BAL supernatants. CCL2 (a) and CXCL1 (b) mRNA expression in lung tissue of WT and BMP-6-deficient mice upon 4 weeks exposure to air or CS. mRNA levels were corrected using a calculated normalization factor based on mRNA expression of three reference genes (GAPDH, TFRC, HPRT-1). Protein levels of CCL2 (c) and TGF-β1 (d) in BAL supernatants of WT and BMP-6 KO mice upon 4 weeks exposure to air or CS, as measured by ELISA. Data are expressed as mean ± SEM (n = 8−12 animals/group; *P < 0.05, **P < 0.01, ***P < 0.001). Data shown are representative of two independent experiments

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