Fig. 4

MUC20 is up-regulated in serum-deprived, hypoxic, and acidic microenvironment. a MUC20 was induced by serum deprivation (0% FBS). b MUC20 was induced by hypoxia (1% oxygen). c MUC20 was induced by acidic condition (pH 6.5). PDAC cells were treated with these different microenvironmental factors for 24 h. The expression of MUC20 was analysed by western blotting. β-actin was used as an internal control. Statistical results for MUC20 signals are shown. Data are presented as mean (n = 3) ± SD. *P < 0.05; **P < 0.01