Fig. 3: AURKA genetic targeting restores chemosensitivity. | Oncogene

Fig. 3: AURKA genetic targeting restores chemosensitivity.

From: Aurora-A kinase oncogenic signaling mediates TGF-β-induced triple-negative breast cancer plasticity and chemoresistance

Fig. 3

a BT-549 cells were cultured under non-adherent conditions for 24 days (three serial passages) to form tertiary mammospheres (MPS). In total, 10,000 cells derived from tertiary MPS were then infected with scrambled or AURKA lenti-shRNAs, treated with 10 ng/ml TGF-β1 in the presence of DMSO (control) or 10 nM docetaxel (DTX) and incubated for 8 days to monitor MPS growth. MPS were labeled with 5 μM Cell Tracker Red CMTPX (Thermo Fisher Scientific, #C34552) for 1 h and the MPS area was measured using the NIH Image-J software. b Graph showing the average of MPS area from three independent experiments (±s.d.). c GFP-tagged SUM149-PT cells were cultured under non-adherent conditions for 24 days (three serial passages) to form tertiary MPS. In total, 10,000 cells derived from tertiary MPS were then infected with scrambled or AURKA lenti-shRNAs and treated with DMSO (control) or 10 nM DTX for 8 days to monitor MPS growth. MPS area was measured using the NIH Image-J software. d Graph showing the average of MPS area from three independent experiments (±s.d.).

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