Fig. 7: Expression of TFAP2A and TFAP2C influences BC cell migration and invasion. | Oncogenesis

Fig. 7: Expression of TFAP2A and TFAP2C influences BC cell migration and invasion.

From: Repression of transcription factor AP-2 alpha by PPARγ reveals a novel transcriptional circuit in basal-squamous bladder cancer

Fig. 7

a Western blotting analysis of TFAP2A and TFAP2C expression in SCaBER cells after siRNA transfection. SiRNAs included Scrambled (negative control), as well as constructs targeting TFAP2A and TFAP2C individually and in combination. b Densitometric analysis of western blotting results for TFAP2A and TFAP2C expression for data depicted in (a). TFAP2A and TFAP2C expression was normalized to GAPDH. Data are expressed as the mean ± S.D from three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001, one-way ANOVA with post hoc multiple comparison (Dunnett). c Representative images following migration and Invasion assays using SCaBER cells transfected with siRNA for Scrambled construct, TFAP2A, TFAP2C, and TFAP2A/TFAP2C. d Relative migration and invasion of SCaBER cells transfected with siRNA. Data are expressed as the mean ± S.D. from three independent experiments. ***p < 0.001, ****p < 0.0001, one-way ANOVA with post hoc multiple comparison (Dunnett). e Western blotting analysis of TFAP2A and TFAP2C protein expression levels in UMUC3 cells overexpressing TFAP2A (left) or TFAP2C (right). Also included is densitometric analysis of western blotting data for TFAP2A and TFAP2C. f q-RT-PCR analysis of TFAP2A and TFAP2C expression in UMUC3 cells overexpressing TFAP2A and TFAP2C. g Migration and Invasion assay of UMUC3 stable cells overexpressing TFAP2A. h Migration and Invasion assay of UMUC3 cell overexpressing TFAP2C. Data are expressed as the mean ± S.D. from three independent experiments. **p < 0.01 (Student’s t test).

Back to article page