Fig. 6 | Signal Transduction and Targeted Therapy

Fig. 6

From: m6A RNA methylation-mediated HNF3γ reduction renders hepatocellular carcinoma dedifferentiation and sorafenib resistance

Fig. 6

HNF3γ inhibits HCC cell proliferation and xenografted HCC growth. a Flow cytometry analysis of HCCLM3 or Huh7 cells infected with Ad-HNF3γ or Ad-Con. b Representative images of proliferating HCCLM3 or Huh7 cells indicated by EdU staining are shown. The nuclei were counterstained with DAPI. EdU+ cells were stained with red immunofluorescence. Scale bars, 25 μm. Results were expressed as means ± SEM. c HCCLM3-xenografted tumor morphology, tumor growth curve, and average tumor weight in Ad-HNF3γ and Ad-Con groups are shown. d Representative images of IHC staining of HNF3γ, Ki67, AFP, CK19, and CD133 in the xenografted tumors are shown. Scale bar, 100 μm. e The expression of hepatocyte-specific genes and differentiation-associated genes in HCCLM3-xenografted tumors were examined by real-time PCR. f Patient-derived HCC xenografts were treated with either AAV-HNF3γ or AAV-GFP. The size of xenografted tumors was measured every week, and the volume was calculated

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