Fig. 3

HFD stimulates Cxcl10-dependent T cell recruitment in tumor-seeded eFats. a The eFats of HFD-treated mice attracted T cells. The eFats were isolated from tumor-seeded mice fed a CD or HFD for 5 days. Mouse splenocytes were cultured in the upper chamber, and the eFats were cultured in the lower chamber of a Transwell system. Twenty-four hours later, the migrated T cells, CD4⁺ T cells, and CD8⁺ T cells were analyzed by flow cytometry (n = 3). b Proliferation rate of T cells cocultured with the eFats from tumor-seeded mice treated with a CD or HFD for 5 days (n = 5). c Heat map showing differentially expressed chemokines in the eFats. mRNA sequencing assays were performed on the eFats isolated from tumor-seeded mice fed a CD or HFD for 5 days. Each sample was pooled from three individual samples. d CXCL10 is required for HFD-induced T cell migration ex vivo. The eFats were isolated from tumor-seeded mice fed a CD or HFD for 5 days. Mouse splenocytes were cultured in the upper chamber, and the eFats were cultured in the lower chamber of a Transwell system. A neutralizing anti-CXCL10 antibody (100 ng ml⁻¹) was added into the lower chamber to block CXCL10 activity. Twenty-four hours later, the migrated T cells in the lower chamber were analyzed by flow cytometry. T cells were defined as CD45⁺CD11b⁻CD3⁺ (n = 5). e Relative mRNA levels of Cxcl10 in the eFats from tumor-seeded WT or Cxcl10 knockout (Cxcl10^−/−) mice fed a CD or HFD for 5 days. nd not detected (n = 4). f, g Infiltration of total T cells, CD4⁺ T cells, CD8⁺ T cells (f), and IFNγ⁺ T cells (g) into the eFats of WT or Cxcl10^−/− mice fed a CD or HFD for 5 days. Each sample was pooled from 3 individual samples (n = 3). h An MC-38-based tumor-seeded model was constructed in WT and Cxcl10^−/− mice. Peritoneal tumor nodes were collected on day 14 and weighed (n = 5). All data are shown as the mean ± s.e.m. (*P < 0.05, **P < 0.01 and ***P < 0.005; ns not significant; Student’s t test)