Fig. 2

Increased expressions of eosinophil-derived mCCL6 in allergen-challenged mice. a Sequence alignment of the mCCL6 with the hCCL23 and hCCL15. Shaded letters indicate residues at each aligned position that are identical to the hCCL23 and hCCL15 specificity. Dashes indicate gaps that were inserted to optimize the alignment. b Schematic timeline and subsequent sample processing of allergic asthma mouse models. c-e Expressions of mCCL6 in BALF supernatant (c), lung tissue (d), and serum (e) measured by ELISA from NS or OVA-challenged mice. Each point represents an individual mouse; data from 4–5 mice per group are plotted as mean ± SEM. f Correlation of mCCL6 protein levels with the number of eosinophils in murine BALF (n = 17, some of the points are overlapped). g Representative histograms of mCCL6 expression in monocytes, eosinophils, and neutrophils from peripheral blood of NS mice. Cells were gated in Fig. S2a. h Representative lung immunofluorescence of eosinophils (tdTomato⁺, red) in OVA-challenged eoCre/R26-tdTomato mice staining with mCCL6 antibody (green) and DAPI (blue). Scale bar, 40 μm, 20 μm. i Representative histograms of mCCL6 expression in BALF eosinophils from OVA-challenged mice compared with NS mice. j Representative cytospin slides of bone marrow exhibited eosinophils (tdTomato⁺, red) in NS or OVA-challenged eoCre/R26-tdTomato mice staining with mCCL6 antibody (green) and DAPI (blue). Scale bar, 40 μm. k Quantitation of mCCL6⁺ percentage among tdTomato⁺ cells in j (n = 6 per group, 3 images per mouse). **P < 0.01; ***P < 0.001 by unpaired t-test