Fig. 3: The iPolony method quantifies infection across a wide range of infection values. | The ISME Journal

Fig. 3: The iPolony method quantifies infection across a wide range of infection values.

From: A single-cell polony method reveals low levels of infected Prochlorococcus in oligotrophic waters despite high cyanophage abundances

Fig. 3

Synechococcus WH8109 was infected by the T7-like cyanophage, Syn5, at different MOIs to test the ability of the polony method to detect differences in percent infection compared to a the MOI in each experiment and to b a culture-based assay for percent infection [37]. Trend lines represent significant linear regressions between percent infection using the iPolony method and MOI values (F = 143.1, R2 = 0.87, p < 0.001) or percent infection based on the lysis-based assay (F = 55.60, R2 = 0.70, p < 0.001).

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