Fig. 3: Analysis of HSCs/progenitor cells.
From: Mice with Calr mutations homologous to human CALR mutations only exhibit mild thrombocytosis
a Proportions of HSCs and progenitors. No differences were found in the frequencies of any progenitors between WT mice (n = 8) and FS mice (n = 8). b Enumeration of colonies and the serial replating capacity of 5 × 104 BM cells from WT (n = 8) and FS (n = 8) mice. FS BM cells did not exhibit enhanced sequential colony-replating capacity. c Serial transplantation assays. WT or FS BM cells (B6-CD45.2) together with competitor WT BM cells (B6-CD45.1) were transplanted at a 1:1 ratio into lethally irradiated recipients (B6-CD45.1) (n = 14 in each group), and then 1 × 106 recipient BM cells were transplanted into a second set of lethally irradiated recipients (B6-CD45.1) (n = 14 in each group). Chimerism of donor-derived CD45.2 + cells in peripheral blood at 16 weeks after the first and second transplantations is shown. All data are presented as the means ± SEM. *P < 0.05; NS, not significant vs. WT cells. A two-tailed Student’s t test was used
