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Reply to Comment on: ‘Hypoxia differently modulates the release of mitochondrial and nuclear DNA’

British Journal of Cancer volume 124, pages 2037–2038 (2021)Cite this article

We thank the Cox group for their comment on this relevant point. We would like to respond to it by adding a number of details and new observations, which we believe will help BJC readers to better appreciate our work1 and provide additional perspective on it.

As indicated in their comments, a recent report by Cox et al.2,3 (published after our own work appeared in BJC) showed a ninefold increase in circulating DNA of mitochondrial origin (cir-mtDNA3) when frozen storage is performed before high-speed centrifugation (i.e. 15,000×g, for 10 min), as compared with frozen storage only performed at the end of the two-step centrifugation process.3

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References

  1. Otandault, A., Abraham, J. D., Al Amir Dache, Z., Khalyfa, A., Jariel-Encontre, I., Forné, T. et al. Hypoxia differently modulates the release of mitochondrial and nuclear DNA. Br. J. Cancer 122, 715–725 (2020)..

  2. Cox, D. R. A., Wong, B. K. L., Yang, L., Yoshino, O., Testro, A., Muralidharan, V. et al. High speed centrifugation before frozen storage of plasma is critical for quantitative analysis of mitochondrial-derived cell free DNA. Clin. Chem. 66, 1111–1114 (2020).

  3. Bronkhorst, A. J., Ungerer, V., Diehl, F., Anker, P., Dor, Y., Fleischhacker, M. et al. Towards systematic nomenclature for cell-free DNA. Hum. Genet. https://doi.org/10.1007/s00439-020-02227-2 (2020).

  4. Al Amir Dache, Z., Otandault, A., Tanos, R., Pastor, B., Meddeb, R., Sanchez, C. et al. Blood contains circulating cell-free respiratory competent mitochondria. FASEB J. 34, 3616–3630 (2020).

  5. Boudreau, L., Duchez, A. C., Cloutier, N., Soulet, D., Martin, N., Bollinger, J. et al. Platelets 106 release mitochondria serving as substrate for bactericidal group IIA-secreted phospholipase A2 to promote inflammation. Blood 14, 2173–2183 (2014).

  6. Meddeb, R., Pisareva, E. & Thierry, A. R. Guidelines for the preanalytical conditions for analyzing circulating cell-free DNA. Clin. Chem. 5, 623–633 (2019).

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Authors and Affiliations

  1. IRCM, Institut de recherche en cancérologie de Montpellier, Inserm U1194, Université de Montpellier, ICM, Montpellier, France

    Alain R. Thierry, Brice Pastor & Zahra Al Amir Dache

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  1. Alain R. Thierry
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  2. Brice Pastor
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  3. Zahra Al Amir Dache
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Contributions

A.T.: drafted, approved the manuscript prior to submission and supervised the work. Z.A.D. and B.P.: drafted, approved the manuscript prior to submission.

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Correspondence to Alain R. Thierry.

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This article does not contain any individual’s identifiable data in any form. None of the material has been published or is under consideration elsewhere, including online journals. All authors have contributed to and agreed on the content of the 76 manuscript.

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Competing interests

The authors declare no competing interests.

Funding information

A.R. Thierry is supported by INSERM. This work was funded by the “SIRIC Montpellier Cancer Grant INCa_Inserm_DGOS_12553”.

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Thierry, A.R., Pastor, B. & Dache, Z.A.A. Reply to Comment on: ‘Hypoxia differently modulates the release of mitochondrial and nuclear DNA’. Br J Cancer 124, 2037–2038 (2021). https://doi.org/10.1038/s41416-021-01288-y

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  • DOI: https://doi.org/10.1038/s41416-021-01288-y

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