Fig. 5: BAP1 reduces tumor growth in vivo.

a–d Nude mice were randomly divided into 2 groups of 10 mice after each being injected with 5 × 10⁶ SK-N-BE2c cells stably expressing empty expression vector (Ctrl) or FLAG-tagged full-length BAP1. Tumor sizes were measured at the indicated time points. Tumor volumes were estimated based on the length and width of the tumors: [mm³] = (length [mm]) × (width [mm])² × 0.5. (V = L × W2 × 0.5). The number of animals with certain tumor volume is presented in d. The mean tumor volume for each treatment group was graphed plus/minus standard errors (*p < 0.05). e Immunohistochemistry analysis of tumor biopsies isolated from control (Ctrl) or BAP1 expressing neuroblastoma cells using H&E or antibodies against BAP1 (1:500), Bcl-2 (1:1000), and cleaved caspase-3 (Asp175, 1:500). Arrowheads indicate cleaved caspase-3 positive cells. f Quantification of the number of cleaved caspase-3 (Asp175) positive cells in percentage visualized in the sections isolated from control or BAP1 expressing neuroblastoma cells (p < 0.05*). g Relative BAP1 expression and its effect on the expression of Bcl-2 and cytochrome C was determined by western blot from tumor tissue. Actin was used as the internal controls for western blotting