Fig. 3: Knocking down LIMK2 promotes CRC progression and metastasis in vivo. | Cell Death & Disease

Fig. 3: Knocking down LIMK2 promotes CRC progression and metastasis in vivo.

From: Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation

Fig. 3

a LIMK2 shRNA-transduced stable SW480 cells were injected subcutaneously into the back of nude mice to evaluate tumor growth (n = 6). Representative figure of tumors formed is shown. b, c Tumor weight and volume in the back of nude mice injected with indicated cells was measured. The data of all primary tumors are expressed as mean ± S.D. Scatter plots of tumor weight derived from indicated cells at 30 d after subcutaneous implantation. d The representative photographs of LIMK2 staining of subcutaneous tumor are shown (magnification, × 200; scale bar: 50 µm). Proliferative ability was indicated by the Ki-67. e Tumor cells were injected into nude mice through the spleen to evaluate the liver homing capacity of cells (n = 6), respectively (the left panel, magnification, × 40; scale bar: 200 µm; the right panel, magnification, × 400; scale bar: 20 µm). The number of metastatic liver nodules in individual mice was counted under the microscope and expressed as mean ± S.D

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