Fig. 8: BP-1-102 maintained the autophagy-related proteins expression in VSMCs. | Cell Death & Disease

Fig. 8: BP-1-102 maintained the autophagy-related proteins expression in VSMCs.

From: A novel STAT3 inhibitor attenuates angiotensin II-induced abdominal aortic aneurysm progression in mice through modulating vascular inflammation and autophagy

Fig. 8

a Autophagy-related proteins expression in VSMCs treated with AngII (10 μM) for 24 or 48 h were evaluated, compared with untreated (mock group) (left panel). VSMCs were treated with BP-1-102 (0.01–0.5 μM) for 24 h (middle panel). VSMCs were treated with AngII (10 μM) + BP-1-102 (0.01 μM-0.5 μM) for 24 h (right panel). Autophagy-related proteins expression were evaluated using western blotting. b Relative protein levels were determined by normalized with β-actin expression in BP-1-102 (0.01–0.5 μM) groups, respectively, and compared with untreated VSMCs (mock) or with BP-1-102 (0.01 μM) group. n = 4, *P < 0.01 compared with Mock. NS non-significant differences compared with Mock. #P < 0.01 compared with BP-1-102 (0.01 μM) group (two-way ANOVA followed by Tukey’s test). c Relative protein levels were determined by normalized with β-actin expression in AngII (10 μM) (24 or 48 h) groups, AngII (10 μM) + BP-1-102 (0.01–0.5 μM) groups, respectively, and compared with untreated VSMCs (mock) or with AngII (10 μM) (24 h). n = 4, *P < 0.01 compared with Mock. NS (red) non-significant differences compared with Mock. #P < 0.01 compared with AngII (10 μM) for 24 h group. NS (blue) non-significant differences compared with AngII (10 μM) for 24 h group (two-way ANOVA followed by Tukey’s test). d Annexin-V-PE/PI staining was performed using flow cytometry to investigate apoptosis of VSMCs treated with AngII (10 μM) for 24 or 48 h and compared with untreated VSMCs (mock, upper left). VSMCs were treated with BP-1-102 (0.01–0.5 μM) and compared with untreated VSMCs (mock, lower left). VSMCs were treated with AngII (10 μM) + BP-1-102 (0.01–0.5 μM) and compared with untreated VSMCs (mock, lower right).

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