Fig. 3: The majority of Neurog2-iN cells are glutamatergic neurons.

a–h Double staining of mCherry protein (a, e) with VGLUT2 mRNA (b, green, pseudo-color) or Gad1 mRNA (f, green, pseudo-color) on sections of the dorsal midbrain from WT mice that were infected with virus AAV–Neurog2/mCherry at 30 DPI. Arrows in merged image (d) indicate mCherry⁺ cells can express VGLUT2. Arrowheads in merged image (h) indicate mCherry⁺ cells did not express Gad1. The nucleus was stained by DAPI (c, g). i–p Double staining of mCherry with GFP from VGLUT2-GFP mice (i–l) or GFP from Gad1-GFP mice (m–p) on sections of the dorsal midbrain that were infected with virus AAV-Neurog2/mCherry at 40 DPI. (l) and (p) are merged images from the mCherry signal (i, m), GFP signal (j, n), and DAPI (k, o), respectively. Arrows indicate mCherry⁺GFP⁺ cells and arrowheads indicate mCherry⁺GFP^- cells (l, p). q–t Represent mCherry (q) colabeled with PV (r) on sections of the dorsal midbrain from WT mice that were infected with virus AAV–Neurog2/mCherry at 30 DPI. Arrows in merged image (t) indicate mCherry⁺PV⁺ cells and arrowheads indicate mCherry⁺PV^- cells. The nucleus is stained by DAPI (s). u, v Statistical data indicate the percentages of colabeled cells from WT mice (a–h, u) and transgenic mice (i–p, v). (a–h) Confocal images 40x (oil); (i–t) Confocal images 20x. Scale bars: 25 μm (a–h) and 50 μm (i–t).