Fig. 5: Combined treatment with VPA and EPZ6438 promotes regenerative responses in the intestinal epithelium after irradiation.
From: Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration
a Schematic diagram of the administration of VPA/EPZ6438 to C57BL/6 mice in the irradiation-induced injury model. b H&E staining of small intestinal crypts at 3 and 5 days post-irradiation (dpi). The green arrows between the two dashed lines show the length of the crypts. Scale bars, 50 μm. c Quantification of crypt number (10 images were analyzed per mouse) and crypt length (10 crypt–villus axes were counted per mouse) at 3 and 5 dpi (n = 6 mice). P values were determined by two-tailed Mann–Whitney test. d Heatmap displaying the expression of the YAP signature in small intestinal crypts with or without VPA/EPZ6438 treatment at 3 dpi. Representative genes are shown on the right. (n = 2 mice). e Heatmap displaying H3K27me3 signals at promoter regions of YAP target genes in small intestinal crypts at 3 dpi. **P < 0.01; ns, not significant. The experiments in b, c, and e were independently repeated at least twice with similar results.
