Abstract
Background
Vitamin D is a modulator of immune functions. Investigations on the mechanisms of vitamin D action and pathogenesis of Hashimoto’s thyroiditis (HT) have revealed that vitamin D can reduce damages to thyroid cells caused by autoreactive immune cells.
Methods
Totally, 48 female patients with HT disease were introduced to the study by endocrinologists. Patients were divided into two major groups of 24 individuals and treated weekly with 50,000 IU of cholecalciferol (vitamin D group) or placebo (placebo group) using oral administration for 3 months. Eventually, 17 of the 24 patients in each group finished the study. Before and after supplementation, frequencies of Th1, Th17, Th2 and Tr1 cells and mean fluorescent intensity (MFI) of the associated cytokines, including IFN-γ, IL-17, IL-4 and IL-10, were assessed using flow cytometry. Furthermore, gene expression of IL-10 was assessed using real-time PCR.
Results
Results of this study showed that cholecalciferol supplementation caused a significant decrease in Th17/Tr1 ratio. The proportion and MFI of Th1, Th2, Tr1 and Th17 cells included no significant changes in vitamin D group, compared to those in placebo group. Expression rate and MFI of IL-10 increased in both groups. This increase was higher in vitamin D group than placebo group with no significance.
Conclusions
In this novel preliminary clinical trial study, supplementation with cholecalciferol in HT patients for 3 months changed the balance of CD4+ T-cell subsets to improve the disease control. However, further studies are necessary to investigate effects of vitamin D on immune functions in HT patients.
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Acknowledgements
The authors acknowledge the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran, and Mazandaran University of Medical Sciences, Sari, Iran, for providing the facilities and funds via grant number 95-01-27-30905. The authors also wish to thank Ms. Salehi for her helpful comments and discussions and Mr. Ghanavati for his assistance in FACS analysis.
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This study was financially supported by grant no. 95-01-27-30905 from Research Administration Department, Tehran University of Medical Sciences (TUMS), Tehran, Iran and grant no. 2353 from Mazandaran University of Medical Sciences, Sari, Iran.
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The current study was approved by the Ethics Committee of Tehran University of Medical Sciences, Tehran, Iran (approval no. IRCT2016110130644N1).
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Written informed consents were signed by all the patients.
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Nodehi, M., Ajami, A., Izad, M. et al. Effects of vitamin D supplements on frequency of CD4+ T-cell subsets in women with Hashimoto’s thyroiditis: a double-blind placebo-controlled study. Eur J Clin Nutr 73, 1236–1243 (2019). https://doi.org/10.1038/s41430-019-0395-z
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DOI: https://doi.org/10.1038/s41430-019-0395-z
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