Fig. 2

Adamts1 is induced by muscle injury and expressed in the macrophages of skeletal muscles. a Time-course monitoring the expression level of Adamts1 in tibialis anterior (TA) muscles isolated from wild-type mice before and after muscle injuries. Adamts1 levels were quantified using RT-qPCR and the injured muscles were compared to the contralateral uninjured muscle (n = 5). b, c Western blots and quantification of ADAMTS1 protein levels in cell lysates from TA muscles before and after muscle injuries compared to Actin control (n = 3 for each time point). d Images from IHC performed on frozen sections prepared from TA muscles 1 day post injury (insets are higher magnification images of a F4/80- and ADAMTS1-positive cell that is present in the lower magnification field). Histological slides were stained using antibodies against ADAMTS1 (red) and F4/80 (green); a marker for macrophages. ADAMTS1 co-localized with macrophages (inset). Scale bar = 10 μm. e Flow cytometry over a time-course monitoring Ly6C in F4/80+cells after muscle injuries. f RT-qPCR measuring the expression levels of Adamts1 in Ly6C+ compared to Ly6C− macrophages. g Representative flow cytometry plots showing ADAMTS1 expression in the macrophage population isolated from injured muscle tissue. *P < 0.05, ***P < 0.001. Error bars represent s.d. Statistical significance tested using paired t-tests