Fig. 4

Basal activity of the HPT axis is not changed in mice with a tanycyte-specific deficiency of Gα_q/11. a tdTomato (gray) was expressed in tanycytes 2 weeks after injecting AAV-Dio2-iCre-2A-GFP in Ai14 reporter mice (scale bar, 100 µm). b Immunofluorescence staining of iCre-2A (white; 2A) and vimentin (green) showed colocalization in tdTomato-positive tanycytes (red; scale bar, 75 µm). c Knockout strategy to generate Gα _q/11 ^tanKO mice. Cre recombinase was transduced by injecting AAV-Dio2-iCre-2A-GFP into the lateral ventricle of Gα _q ^fl/fl::Gα ₁₁ ^−/− mice. AAV-Dio2-GFP injected Gα _q ^fl/fl::Gα ₁₁ ^−/+ into the lateral ventricle were used as control (Gα _q/11 ^Con) d–f Basal plasma concentrations of TSH (d; p = 0.59), T4 (e; p = 0.75), and T3 (f; p = 0.34) 3 weeks after inducing the knockout by injecting rAAV (Gα _q/11 ^Con, gray; Gα _q/11 ^tanKO, blue). g TRH-DE activity in Gα _q/11 ^Con mice (gray) and Gα _q/11 ^tanKO (blue) 3 weeks after rAAV injection. **p = 0.0093 (two-tailed Student’s t-test); mean ± S.E.M.; n, as indicated