Fig. 3

NAbs PGT125, 126, 128, and 130 bind avidly to NIT82B, a neoglycoconjugate presenting an Rv3-derived mimic of oligomannose. a The listed conjugates are those from Fig. 2. The conjugates (69–72 kDa) were coated as solid-phase antigen onto ELISA plate wells at 5 µg ml⁻¹ and assayed for recognition by PGT125, 126, 128, and 130 (starting at 2 µg ml⁻¹). All antibodies were tested as IgGs. The data represent the means from two independent experiments, with error bars omitted for clarity. GraphPad Prism (version 7.02) was used to determined EC₅₀ values (0.1–5 nM). b To assess the importance of valency for antibody binding, conjugate NIT82B was coated onto ELISA plates (5 µg ml⁻¹) and antibody binding assessed in the presence of the unconjugated 3-aminopropyl glycosides NIT68A (presented on NIT82A), NIT68B (presented on NIT82B) or NIT70A (presented on NIT83A), or the NIT82B glycoconjugate as a comparator (dashed line). The results, obtained from one experiment, show that the antibodies are inhibited best by the NIT82B conjugate