Fig. 2
MCJ deletion protects against APAP hepatotoxicity in vivo. WT (n = 6) and MCJ KO mice (n = 6) were treated with APAP 360 mg/kg for 48 h. Liver damage was evaluated by a hematoxylin and eosin (H&E) and b TUNEL staining in liver sections. c, d Serum alanine aminotransferase (ALT) levels in WT and MCJ KO fed animals (c) and starved animals at 24 h after APAP (d). e Inflammation assessed by F4/80 staining in liver. f Total GSH levels measured by HPLC-MS in WT and MCJ KO livers after 6 h of APAP treatment (n = 5). g GSSG/GSH levels measured by HPLC-MS in WT and MCJ KO livers. h JNK activation by western blotting in WT and MCJ KO liver extracts after 1 h of APAP treatment (n = 5). i ROS in vivo measured by dihydroethidium (DHE) staining in liver sections. Scale bar corresponds to 100 μm. Values are represented as mean ± SEM. *P < 0.05 (Student’s t test) (MCJ KO vs. WT)
