Fig. 4

H&E stained from breast specimen of patients present invasive ductal carcinoma. a, e Meanwhile H&E staining results from the lymph nodes of the patients presented no trace of tumor cells (b, f). Multilevel IHC staining from the lymph nodes of the patents revealed the presence of at least one tumor cell sharply expressing the PCK (c, g) or Vimentin (d, h) for both suspicious patients. i Diagnostic chart of Metas-Chip showing “final electrical response of the fourth hour after adding the lymph sample to Metas-chip/Initial electrical response before adding the sample” for all patients. The scale bars are 25 µm in length