Fig. 5
From: Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors
Combinations of −10 and −35 sites with large dynamic ranges are compatible with diverse regulatory sequences. a Experimentally measured transcription rates of a collection of engineered two-input hybrid promoters that use the dE, dF, bD, or eG combination of −10 and −35 sites. The promoters contain operator sites for one of the five different transcriptional activators (AraC, XylR, RhlR, LasR, or LuxR) and one of the two transcriptional repressors (LacI or TetR). Each promoter was tested in all four possible combinations of inducers for its respective activator and repressor. The AND gate is considered well behaved if the promoter is active only in the presence of both inducers. b Experimentally measured transcription rates of a collection of engineered three-input hybrid promoters that use the dE, dF, bD, or eG combination of −10 and −35 sites. These promoters contain operator sites for one of the five different activators (AraC, XylR, RhlR, LasR, or LuxR) and the operator sites for both LacI and TetR. Each promoter was tested in all eight possible combinations of inducers for its respective activator and repressor. Transcription should occur if and only if the inducers for all three transcription factors are present. Experiments were performed with biological triplicates. Experimental data for negative controls are available in the Supplementary Information (Supplementary Figure 2). Experimental data are available in Supplementary Data 6–8
