Fig. 1
Immunofluorescence staining of archival brain tissues. a Upper panel: comparison of formalin-fixed human cerebellar tissue immersed in PBS (left), OPTIClear (middle) and OPTIClear after 5 days of SDS delipidation at 55 °C (right). Lower panel: comparison of 5 mm-thick, formalin-fixed human striatum tissue block after 3.5 months of SDS delipidation at 55 °C, before (left) and (after) OPTIClearing. b Colour depth-coded, Z-stack image of a block of 2 mm-thick cingulate cortex that has been formalin-fixed for 50 years, stained with antibodies against GFAP with an imaging depth of 125.57 µm. The tissue was cleared after SDS treatment for 4 months at 55 °C and cleared in OPTIClear. Scale bar = 50 µm. c Z-stack image of a piece of formalin-fixed, paraffin-embedded midbrain tissue immunostained for GFAP (red) and counterstained with DAPI (blue) after dewaxing and rehydration (Z-stack depth = 20.77 µm)
